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本研究以沙打旺(Astrgalus adsurgens)感病品种宁夏彭阳为试验材料,接种沙打旺埃里砖格孢(Embellisia astragali)后分别施加外源NO及H2O2清除剂抗坏血酸,测定H2O2含量和H2O2代谢酶SOD、POD和CAT活性,以此来探讨H2O2在由NO诱导的防御反应中发挥的作用。与对照相比,外源NO显著降低(P<0.05)沙打旺发病率和病情指数,在开始阶段,外源NO可以诱导内源H2O2产生,在后期,当H2O2浓度相对较高,外源NO会延迟H2O2积累,但用NO和H2O2清除剂同时处理H2O2含量与对照并无显著差异(P>0.05)。NO处理能够诱导SOD、POD和CAT活性增加,H2O2清除剂抑制了NO对3种酶的调控作用。施加外源NO能增强沙打旺抗病性,NO通过调控内源H2O2含量发挥作用。
In this study, Astrgalus adsurgens susceptible cultivar Pengyang was used as experimental material. Exogenous NO and H2O2 scavenger ascorbic acid were respectively inoculated with Embellisia astragali, and the content of H2O2 and the content of H2O2 Metabolism enzymes SOD, POD and CAT activity, in order to explore the role of H2O2 in NO-induced defensive response. Compared with the control, exogenous NO significantly reduced (P <0.05) the incidence and severity index of strychnine. In the initial stage, exogenous NO induced endogenous H2O2 production. In the later stage, when the concentration of H2O2 was relatively high, exogenous NO delayed the accumulation of H2O2. However, there was no significant difference (P> 0.05) between H2O2 content and NO and H2O2 scavengers. NO treatment could induce the increase of SOD, POD and CAT activity, H2O2 scavenger inhibited the regulation of NO on three enzymes. Applying exogenous NO can enhance the resistance of Astragalus adsurgens, NO play a role by regulating the content of endogenous H2O2.