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方便、快捷、准确地对转基因小麦中的bar基因进行检测,对于筛选纯合稳定转基因植株、获得无筛选标记转基因植株、鉴定常规小麦品种和商品小麦中的bar基因成分等具有一定价值。本试验对叶片涂抹、植株喷洒、培养基添加除草剂3种方法鉴定转bar基因小麦植株的效果进行了比较,表明3种方法都能很好鉴定转基因小麦中的bar基因,叶片涂抹200mg/LLiberty鉴别的准确性高于PCR检测,植株喷洒Basta的适宜浓度为100mg/L,喷洒Liberty的适宜浓度为150mg/L,培养基添加Bialaphos的适宜浓度为5～8mg/L。叶片涂抹和植株喷洒除草剂方法受环境条件影响较大,区别转基因植株和非转基因植株的标准不够明确。相比之下,成熟胚离体培养除草剂筛选不受外界环境条件的影响,具有鉴定效果直观明了、操作简单、试验周期短等优点,在检测小麦转入或飘入的bar基因方面具有潜在应用前景。
The detection of bar gene in transgenic wheat is convenient, fast and accurate. It has certain value for screening homozygous stable transgenic plants, obtaining transgenic plants without screening markers, and identifying the bar gene components in conventional wheat varieties and commercial wheat. In this experiment, the effects of leaf painting, spraying plants and adding herbicide on the identification of bar transgenic wheat plants were compared. The results showed that all the three methods could well identify the bar gene in transgenic wheat with 200mg / LLiberty The accuracy of the identification was higher than that of PCR. The suitable concentration of Basta for plant spraying was 100mg / L, the suitable concentration of Liberty for spraying was 150mg / L, the suitable concentration of Bialaphos for medium was 5-8mg / L. Leaf smear and herbicide spraying methods are more affected by environmental conditions, the standard is not clear enough to distinguish between transgenic and non-transgenic plants. In contrast, screening of mature embryos in vitro culture herbicide is not affected by external environmental conditions, with the identification effect is intuitive, simple operation, short test period, etc., in detecting the wheat into or floating bar genes potential Application prospects.