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为了开发一种有效防治烟草青枯病的微生物制剂，对烟草青枯病田间小区试验防效最好的３个细菌菌株湘１－２，湘２－３，２－轻－９进行了发酵培养条件研究．结果表明，湘１－２在３０～３２℃，ｐＨ７．１３～８．０，振荡培养２４ｈ时产菌量最多，１０～２４ｈ为对数生长期，温度低于４℃和ｐＨ≤５．３２不生长；湘２－３在３０～３２℃，ｐＨ７．１３～９．１８，振荡培养２２ｈ产菌量最多，４～２２ｈ为对数生长期，温度低于４℃，ｐＨ≤４．６８不生长也不死亡；２－轻－９在２８～３２℃，ｐＨ７．６５～８．０，振荡培养２２ｈ产菌量最多，１０～２２ｈ为对数生长期，低于４℃，ｐＨ≤５．７４不生长也不死亡．同时采用几种价廉易得的原料组配了７个培养基组合，用于培养这３个菌株．湘１－２较难培养，只在４＃，５＃培养基中能生长且生长不太好；湘２－３在７个培养基中都能生长，但在２＃培养基中生长不太好；２－轻－９在７个培养基中都能生长，但在７＃培养基中生长较差
In order to develop an effective microbial control agent against tobacco bacterial wilt, three bacterial strains Xiang 1-2, Xiang 2-3, 2-Xia9 Conditions research. The results showed that Xiang 1-2 was the most productive strain at 30 ~ 32 ℃, pH7.13 ~ 8.0 and shaking culture for 24h, the logarithmic growth phase was 10 ~ 24h, the temperature was lower than 4 ℃ and pH≤5.32 No growth; Xiang 2-3 at 30 ~ 32 ℃, pH7.13 ~ 9.18, the highest 22h shaking culture bacteria, 4 ~ 22h logarithmic growth phase, the temperature below 4 ℃, pH ≤ 4.68 not The growth of 2-MS-9 was at 28 ~ 32 ℃, pH7.65 ~ 8.0, and the maximum amount of bacteria was produced by shaking culture for 22h. The logarithmic growth phase was 10 ~ 22h, and the pH was lower than 4 ℃. 74 does not grow nor die. At the same time using several inexpensive raw materials with a combination of seven medium combinations for the culture of these three strains. Hunan 1-2 harder to grow, only in 4 #, 5 # medium can grow and growth is not very good; Hunan 2-3 can grow in 7 medium, but growth in 2 # medium is not too Good; 2 - light -9 can grow in all 7 medium, but poor growth in 7 # medium