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集落刺激因子(CSF)是CFU-C体外培养中的一个必不可少的条件,它对培养结果的好坏起着决定性的作用。在狗的骨髓细胞培养中,异体血清是目前普遍采用的一种CSF,但因制备方法不同,其刺激活力可有显著差别。为了选定CSF活力较强的狗血清,我们进行了以下实验研究。材料与方法一、CSF的制备 1.狗血清选成年狗,分别于照前1天和照后1、7、11、18、21、25、30天采静脉血10ml,分离血清,置-20℃冰冻保存。照射条件为~(60)Co γ线3.0戈瑞全身照射。
Colony stimulating factor (CSF) is an essential condition for CFU-C in vitro culture, which plays a decisive role in the quality of culture. In dog bone marrow cell culture, allogeneic serum is currently a commonly used CSF, but due to different preparation methods, the stimulation of vitality can be significantly different. In order to select a highly active dog serum CSF, we conducted the following experimental study. Materials and methods First, the preparation of CSF 1. Dog serum selected adult dogs, respectively, in the day before and after taking 1,7,11,18,21,25,30,30 days were collected venous blood 10ml, serum was separated, set -20 ℃ frozen preservation. Irradiation was ~ (60) Co γ γ 3.0 Gy. Whole body irradiation.