论文部分内容阅读
目的观察银杏叶提取物预处理对兔心肌缺血再灌注前后 ＰＭＮ（中性粒细胞）的活性变化过程。了解银杏叶提取物保护心肌是否与影响中性粒细胞活化作用有关、方法取３０只日本大耳白兔随机均分为３组，假手术对照组、缺血再灌注组和银杏叶提取物预处理用药组。用药方法；用自制的银杏叶提取物注射剂（总黄酮含量５ｍｇ／ｍｌ）５ｍｌ静滴给药。分别于术前和冠脉结扎缺血１ｈ后采血用布氏显微镜对比观察白细胞活化状态的变化。检测白细胞表面粘附分子 ＣＤ；，／ＣＤ_１８表达度、血浆ＮＯ（一氧化氮）、 ＥＴ（内皮素）、ＭＤＡ（丙二醛）、ＣＴｎｌ（肌钙蛋白１），电镜下心肌组织起微结构的变化。结果在布氏显微镜下白细胞活化状态的变化是；冠脉结扎前活化的中性粒细胞（ＰＭＮ）不超过５％，结扎１ｈ后活化的白细胞达于４０％～６０％，而ＧＢＥ预处理组结扎１ｈ后活化的白细胞不超过１８％。表面粘附分子 ＣＤ１１／ＣＤ_１８表达率的变化趋势与布氏显微镜下白细胞活化状态的变化相一致。心肌缺血再灌注后血浆ＭＤＡ、ＣＴｎｌ、ＥＴ含量明显升高，血浆ＮＯ明显减少。心肌细胞线粒体肿胀、嵴减少，内皮细胞肿胀，血管腔内充满红细胞、白细胞。ＧＢＥ预处理组血浆ＭＤＡ、ＣＴｎｌ、ＥＴ含量明显低于?
Objective To observe the changes of activity of PMN (neutrophil) before and after myocardial ischemia reperfusion in rabbits by pretreatment with Ginkgo biloba extract. Understanding whether Ginkgo biloba extract protects myocardium is related to neutrophil activation, 30 Japanese rabbits were randomly divided into 3 groups: sham-operated control group, ischemia-reperfusion group, and Ginkgo biloba extract pre-treatment. Treatment group. Method of administration: 5 ml of a homemade Ginkgo biloba extract injection (total flavonoid content 5 mg/ml) was intravenously administered. Preoperatively and ligation of coronary artery ligation for 1 h after blood collection, Büchner microscopy was used to observe changes in leukocyte activation. To detect the expression of CD;,/CD_18, plasma NO (nitric oxide), ET (endothelin), MDA (malondialdehyde), CTnl (troponin 1), and electron microscopy. The change. Results The change of leukocyte activation state under Büchner microscope was that the number of activated neutrophils (PMN) before coronary artery ligation was less than 5%, and that after 1 hour of ligation was 40% to 60% of activated leukocytes, while GBE pretreatment group The activated leukocytes did not exceed 18% after 1 h of ligation. The change trend of the CD11/CD18 expression rate of the surface adhesion molecules was consistent with the change of the leukocyte activation state under the Brookfield microscope. After myocardial ischemia and reperfusion, plasma MDA, CTnl, and ET levels increased significantly, and plasma NO decreased significantly. Myocardial mitochondria are swollen, maggots are reduced, endothelial cells are swollen, and blood vessels are filled with red and white blood cells. The plasma MDA, CTnl, and ET levels in the GBE pretreatment group were significantly lower than those in the GBE group.