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基于构建获得的云南切梢小蠹转录组数据库,利用MISA(MicroSatellite)软件对其微卫星进行了高通量发掘。在32595681 bp的转录组序列中,共发掘获得1098个微卫星序列。在这些微卫星序列中,单核苷酸和三核苷酸微卫星重复单元最为丰富,分别为420和482个;六核苷酸的微卫星重复单元的丰度最小,仅有3个。就重复序列而言,以A/T单碱基重复序列最多,有367个;其次是AAT/ATT和ATC/ATG三碱基序列,有83个;最少的是CG/CG二碱基序列,有2个。研究结果为开发高多态性微卫星引物来进行云南切梢小蠹种群遗传结构、种群遗传多样性、功能基因组学等研究奠定了基础。
Based on the constructed transcriptome database of C. minutus, high-throughput microsatellites were screened using MISA (MicroSatellite) software. In 32595681 bp transcriptome sequences, a total of 1098 microsatellite sequences were found. Among these microsatellite sequences, the mononucleotide and trinucleotide microsatellite repeat units were the most abundant, which were 420 and 482, respectively. The six-nucleotide microsatellite repeat units had the smallest abundance of only three. In terms of repeats, A / T had the largest number of single base repeats with 367 AAT / ATT and ATC / ATG triad sequences, with 83; the least was the CG / CG dinucleotide sequence, There are two. The results laid the foundation for the research on the genetic structure, population genetic diversity, functional genomics and other aspects of the development of high polymorphic microsatellite primers.