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目的了解良恶性淋巴上皮性病变与埃泼斯坦－巴尔（ＥＢ）病毒的关系。方法采用原位杂交、聚合酶链反应（ＰＣＲ）及免疫组化ＬＳＡＢ法对涎腺１８例恶性淋巴上皮病变（ＭＬＥＬ）、１４例良性淋巴上皮病变（ＢＬＥＬ）进行ＥＢＶ编码的ＲＮＡ（ＥＢＥＲ１）、ＥＢＶＢａｍＨ１Ｗ片段及ＥＢＶ潜在膜蛋白（ＬＭＰ１）和潜在膜抗原（ＥＢＮＡ２）检测。结果（１）本组１８例ＭＬＥＬＥＢＥＲ１和ＥＢＶＢａｍＨ１Ｗ片段均为阳性，１４例ＢＬＥＬ则为阴性。（２）１８例ＭＬＥＬＬＭＰ１检出率７７．８％（１４／１８），ＥＢＮＡ２未检出（０／９）。（３）ＭＬＥＬ间质淋巴细胞多数为Ｔ细胞，ＢＬＥＬ间质多为Ｂ细胞。（４）本组统计２１６例涎腺癌中有３７例ＭＬＥＬ，发生率为１７．１％。结论本结果表明ＥＢＶ与ＭＬＥＬ存在密切关系，很可能在其发病中起着重要作用。文中还对ＢＬＥＬ与ＥＢＶ的关系、ＢＬＥＬ与ＭＬＥＬ的关系进行了讨论。
Objective To investigate the relationship between benign and malignant lymphoepithelial lesions and Epstein-Barr virus (EB). Methods Epstein-Barr virus (EBER1) RNA was amplified from 18 cases of MLEL and 14 cases of benign lymphoepithelial lesions (BLEL) by in situ hybridization, polymerase chain reaction (PCR) and immunohistochemistry. EBVBamH1W fragment and EBV potential membrane protein (LMP1) and potential membrane antigen (EBNA2) detection. Results (1) The 18 cases of MLELEBER1 and EBVBamH1W fragments were positive, 14 cases of BLEL was negative. (2) The detection rate of MLELLMP1 in 18 cases was 77.8% (14/18), while EBNA2 was not detected (0/9). (3) The majority of MLEL interstitial lymphocytes are T cells, while BLEL is mostly B cells. (4) The group of 216 cases of salivary gland cancer in 37 cases of MLEL, the incidence was 17.1%. Conclusion The results suggest that EBV is closely related to MLEL and may play an important role in its pathogenesis. The article also discusses the relationship between BLEL and EBV, the relationship between BLEL and MLEL.