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Methods: The target sequence of ER RNA covering exon4～6(1082～1520bp) was amplified in 7 clinical human breast cancer tissues by reverse transcription and polymerase chain reaction (RT-PCR) techniques.Results: PCR products were transferred to nitrocellulose membranes and hybridized using a [r-32P]-ATP labeled ER 29 oligonulceotide probe representing the antisense strand of the ER Cdna sequence 1271～1299. Specific bands were found at 438 and 300 base pairs in two tumors. The 300 base pair of PCR product was recovered from ER+/PR+ and ER+/PR- tumor, respectively.Conclusion: Dideoxy sequence analysis revealed that they contained the variant ER completely missing exon 5.