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Objective: To study the effect of ligustrazine nanoparticles nano spray(LNNS) on transforming growth factor β(TGF-β)/Smad signal protein of rat peritoneal mesothelial cells(RPMC) induced by tumor necrosis factor α(TNF-α), and the anti-adhesion mechanism of LNNS in the abdominal cavity. Methods: The primary culture and subculture of rat peritoneal mesothelial cells(RPMC) was processed by trypsin digestion method in vitro. The third generation was identified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group(2.5 mg/L); RPMC treated by a medium-dosage LNNS group(5 mg/L); and RPMC treated by a high-dosage LNNS group(10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fibronectin, collagen Ⅰ(COL-Ⅰ), TGF-β m RNA, and Western blot method to test the Smad protein 7 expression of RPMC. Results: Compared with the blank group, a significant elevation in fibronectin(FN), COL-Ⅰ and TGF-β m RNA expression of RPMC were observed in the control group(P<0.05). Compared with the control group, LNNS suppressed the expressions of FN, COL-Ⅰ and TGF-β m RNA in a concentrationdependent manner(P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose(P<0.05). Conclusions: TNF-α may induce changes in RPMC’s viability, leading to peritoneal injury. LNNS could reverse the induction of fibrosis related cytokine FN, COL-Ⅰ and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.
Objective: To study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor β (TGF-β) / Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor α (TNF-α) anti-adhesion mechanism of LNNS in the abdominal cavity. Methods: The primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identified for experiment and divided into 5 groups: a blank RPMC treated with a low-dosage LNNS group (2.5 mg / L); RPMC treated with a medium-dosage LNNS group (5 mg / L); and Reverse transcription-polymerase chain reaction was applied to test the expression of fibronectin, collagen I (COL-I), TGF-β m RNA, and Western blot method to test the Smad protein 7 expression of RPMC. Results: Compared with the blank group, as (P <0.05). Compared with the control group, LNNS suppressed the expressions of FN, COL-I and TGF (P <0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose (P <0.05). Conclusions: TNF -α may induce changes in RPMC’s viability, leading to peritoneal injury. LNNS could reverse the induction of fibrosis related cytokine FN, COL-I and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.