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目的:建立血清胸腺因子的含量测定方法。方法:高效液相色谱法,采用HILIC色谱柱;以0.05 mol·L-1磷酸缓冲液(p H 6.0)-乙腈(55∶45)为流动相;流速1.0 m L·min-1;检测波长210 nm。结果:进样量在1.5~12μg的范围内与峰面积呈良好的线性关系(r=1);最低检出限为10 ng;平均回收率为99.7%,RSD(n=9)为1.0%。结论:该方法简便、准确,对血清胸腺因子的含量测定结果可靠。
Objective: To establish a method for the determination of serum thymus factor. METHODS: High performance liquid chromatography (HPLC) was performed on a HILIC column with 0.05 mol·L-1 phosphate buffer (p H 6.0) -acetonitrile (55:45) as mobile phase at a flow rate of 1.0 m L · min- 210 nm. Results: The calibration curve showed a good linear relationship with the peak area in the range of 1.5 ~ 12μg (r = 1). The detection limit was 10 ng. The average recovery was 99.7% and the RSD (n = 9) was 1.0% . Conclusion: The method is simple and accurate, and the determination of serum thymus content is reliable.