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目的：探讨白细胞介素６（ＩＬ６）在多发性骨髓瘤（ＭＭ）中过度表达的机制。方法：采用多聚甲醛分别固定骨髓瘤细胞ＫＭ３和骨髓基质细胞，再将其置于同一体系中共同培养，用Ｂ９细胞增殖试验测量培养上清中ＩＬ６活性。结果：骨髓基质细胞及骨髓瘤细胞均能自发分泌ＩＬ６，两种细胞经固定后几乎均不能分泌ＩＬ６。固定的骨髓瘤细胞与未经固定的骨髓基质细胞共培养后，可使共培养上清中的ＩＬ６活性明显增加，同时固定后的骨髓基质细胞与未固定的骨髓瘤细胞共培养后亦可使后者分泌ＩＬ６增加，其中ＭＭ患者的骨髓基质细胞较正常对照组骨髓基质细胞与骨髓瘤细胞共培养后上清中ＩＬ６活性增加更明显（Ｐ均＜０．０１）。结论：ＭＭ患者ＩＬ６过度产生的机制存在二相性，即骨髓瘤细胞和骨髓基质细胞均可过度表达ＩＬ６，参与ＭＭ的发病。
Objective: To investigate the mechanism of overexpression of interleukin 6 (IL-6) in multiple myeloma (MM). METHODS: A paraformaldehyde was used to fix myeloma cells KM3 and bone marrow stromal cells, and then they were placed in the same system for coculture. The activity of IL-6 in the culture supernatant was measured by B9 cell proliferation assay. RESULTS: Both bone marrow stromal cells and myeloma cells secreted IL-6 spontaneously, and both cells could hardly secrete IL-6 after fixation. After fixed myeloma cells were co-cultured with unfixed bone marrow stromal cells, IL-6 activity in the co-cultured supernatant was significantly increased, and the fixed bone marrow stromal cells were co-cultured with unfixed myeloma cells. The IL 6 secreted by the latter can be increased, and the activity of IL-6 in the supernatant of the MM patient’s bone marrow stromal cells was significantly higher than that of the normal control group (P<0.01). Conclusion: The mechanism of overproduction of IL-6 in MM patients is biphasic, that is, myeloma cells and bone marrow stromal cells can overexpress IL-6 and participate in the pathogenesis of MM.