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TCRαβ + CD4-CD8- (TCR+ DN) thymocytes at different developmental periods, i.e. after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb significantly promoted proliferation of TCRαβ+ DN cells generated after 18 days of culture in FTOC, whereas the cells generated after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRαβ+ DN cells at day 9 of FTOC to differentiate into TCRαβ+ CD4+ /CD8 + SP cells without detectable transitional stage of TCRαβ+CD4 + CD8+(DP) cells. In contrast, fewerTCRαβ+ DN cells generated after 18 days of FTOC were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRαβ+ DN cells. In addition, TCRαβ+ DN cells were shown to be less susceptible to apoptosis compared with the other
TCRαβ + CD4-CD8- (TCR + DN) thymocytes at different developmental periods, ie after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb by promoted proliferation of TCRαβ + DN cells generated after 18 days of culture in FTOC, whereas the cells were produced after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRαβ + DN cells at day 9 of FTOC to differentiate into TCRαβ + CD4 + / CD8 + SP cells without detectable transitional stage of TCRαβ + CD4 + CD8 + (DP) cells. In contrast, fewer TCRαβ + DN cells were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRαβ + DN cells. In addition, TCRαβ + DN cells were shown to be less susceptible to apoptosis compa red with the other