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目的 探讨 G蛋白 β3亚基 ( GNB3)基因 C82 5 T多态性与中国人群出血性脑卒中之间的关系。方法 利用 PCR和分子杂交技术对该位点在中国人群出血性脑卒中患者 ( 2 0 2人 )中的分布进行检测和分析 ,并与无脑血管病变的人群 ( 190人 )进行比较。结果 C82 5 T多态性位点在两组人群中的分布均符合 Hardy- Weinberg遗传平衡定律。CC、CT、TT3种基因型在病例组中分布频率分别为 2 7.2 3%、4 3.0 7%和 2 9.70 % ,对照组中分别为 2 8.95 %、4 8.4 2 %和 2 2 .6 3% ;两组人群 82 5 T等位基因频率分别为 5 1.2 4 %和 4 6 .84 % ,分布均无统计学明显差异 ( P>0 .0 5 )。结论 GNB3基因 C82 5 T多态性不是中国人群出血性脑卒中的分子遗传学标记物
Objective To investigate the relationship between the G82b polymorphism of GNB3 gene and hemorrhagic stroke in Chinese population. Methods The distribution of the locus in hemorrhagic stroke patients (202 patients) in Chinese population was detected and analyzed by PCR and molecular hybridization and compared with those without cerebrovascular disease (190). Results The distribution of C82 5 T polymorphism loci in both groups were in accordance with the Hardy-Weinberg law of genetic equilibrium. The distribution frequency of CC, CT and TT genotypes was 2 7.23%, 4 3.07% and 2. 9.70% respectively in the case group, while the control group was 2 8.95%, 4 8.42% and 22.6% . There was no significant difference in the distribution of 82 T alleles between the two groups (5 1.2 4% and 46.84% respectively) (P> 0.05). Conclusion GNB3 gene C82 5 T polymorphism is not a molecular genetic marker for hemorrhagic stroke in Chinese population