论文部分内容阅读
目的 观察病毒性脑炎 /脑膜炎患儿急性期及恢复期脑脊液神经元烯醇化酶 (NSE)、S 10 0 β蛋白水平的变化 ,并探讨其临床意义。方法 1997年 1月至 2 0 0 3年 3月兰州医学院第一附属医院 6 8例病毒性脑炎 /脑膜炎患儿作为观察组 ,对照组选择同期住院的非中枢神经系统疾病患儿 2 5例。NSE采用竞争性放射免疫分析法测定 ,S 10 0 β采用酶联免疫双抗体夹心法测定。 结果 急性期单纯疮疹病毒 Ⅰ (HSV Ⅰ )、HSV Ⅱ、EBV感染患儿脑脊液NSE及S 10 0 β蛋白质量浓度较对照组明显增高 (P <0 0 1) ,柯萨奇B组病毒、埃可病毒感染患儿脑脊液NSE及S 10 0 β蛋白质量浓度较对照组增高 (P <0 0 5 ) ,且NSE及S 10 0 β蛋白质量浓度与惊厥发作次数、意识障碍程度、头颅CT脑组织低密度范围呈正相关 (P <0 0 1或P <0 0 5 )。恢复期HSV I、HSV II、EBV感染患儿脑脊液NSE及S 10 0 β蛋白质量浓度仍较对照组增高 (P <0 0 1,P <0 0 5 ) ,且NSE、S 10 0 β蛋白质量浓度与预后程度呈负相关 (P <0 0 1,P <0 0 5 ) ,特别是与恢复期脑脊液NSE及S 10 0 β蛋白质量浓度关系密切。急性脑炎患儿在急性期及恢复期脑脊液NSE及S 10 0 β蛋白质量浓度较对照组明显增高 ,急性脑膜炎急性期除HSV Ⅰ、EBV感染患儿增高外 ,其余与对照
Objective To observe the changes of cerebrospinal fluid neuron enolase (NSE) and S 10 0 β protein in acute and convalescent patients with viral encephalitis and meningitis and to investigate its clinical significance. Methods From January 1997 to March 2003, 68 children with viral encephalitis / meningitis in the First Affiliated Hospital of Lanzhou Medical College were selected as the observation group. The control group was treated with non-central nervous system diseases 5 cases. NSE was measured by competitive radioimmunoassay, and S 10 0 β was measured by enzyme-linked immunosorbent assay. Results The concentration of NSE and S 10 0 β in cerebrospinal fluid of children with HSV Ⅰ, HSV Ⅱ and EBV infection in acute stage were significantly higher than those in control group (P <0.01), Coxsackie B virus, The concentration of NSE and S 10 0 β protein in cerebrospinal fluid of children with echovirus infection was higher than that of the control group (P <0.05), and the protein concentration of NSE and S 10 0 β and the number of seizures, the degree of disturbance of consciousness, Tissue density was positively correlated (P <0.01 or P <0.05). The concentrations of NSE and S 10 0 β in cerebrospinal fluid of patients with convalescent HSV I, HSV II and EBV infection were still higher than those of control group (P <0.01, P <0.05), and the protein content of NSE, S 10 0 β There was a negative correlation between the concentration and the degree of prognosis (P <0.01, P <0.05), especially the concentration of NSE and S 10 0 β in cerebrospinal fluid during convalescence. Acute encephalitis in acute and convalescent cerebrospinal fluid NSE and S 10 0 β protein concentration was significantly higher than the control group, acute meningitis in addition to HSV Ⅰ, EBV infection in children with increased, the rest with the control