丹参酮对人白细胞趋化性影响的观察

• 作 者: 高骥援 王淑芬 张克坚 王常慧
• 机 构: 中国医科大学病理生理教研室,中国医科大学病理生理教研室,中国医科大学病理生理教研室,中国医科大学病理生理教研室,
• 出 处: 中西医结合杂志
• 发表日期: 1985年11期
• 关键词: 白细胞趋化性 丹参酮 随机运动 抗炎效应 体外法 抗炎药物 孵育时间 人白细胞 玻片法 实验性研究

本文应用琼脂糖玻片法进一步证实了丹参酮的抗炎效应,并探索了应用体外法白细胞趋化性实验进行抗炎药物筛选的可行性。研究表明,当健康人白细胞(5×10~7/ml)与50ng/ml 丹参酮共同孵育1小时时,可使白细胞趋化性发生明显抑制;而对白细胞的随机运动无影响。若孵育时间持续19小时,则5 ng/ml 丹参酮足以使白细胞趋化性及随机运动均发生有意义的抑制。

In this paper, the anti-inflammatory effect of tanshinone was further confirmed by agarose slide method, and the feasibility of using in vitro leukocyte chemotaxis assay to screen anti-inflammatory drugs was explored. Studies have shown that when healthy human leukocytes (5×10~7/ml) are incubated with 50ng/ml tanshinone for 1 hour, leukocyte chemotaxis can be significantly inhibited; however, there is no effect on the random movement of leukocytes. If incubation lasts for 19 hours, 5 ng/ml tanshinone is sufficient to cause significant inhibition of leukocyte chemotaxis and random movement.