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目的观察雷公藤不同提取部位对小鼠肝肾毒性损伤的作用。方法提取分离雷公藤不同部位,给小鼠分别灌服5、10 d,检测给药后小鼠体内的肝肾损伤相关功能指标,研究雷公藤的肝肾损伤较明显的部位。结果给药5、10 d后,雷公藤醇提物部位、乙酸乙酯部位和正丁醇部位可导致小鼠血中谷丙转氨酶和谷草转氨酶的升高;给药5 d后,乙酸乙酯高剂量组和正丁醇高剂量组以及醇提物高、低剂量组的尿素氮(BUN)值升高,但是对血肌酐(Scr)影响不大;给药10 d后,正丁醇高剂量组以及醇提物高、低剂量组的BUN升高,醇提物高、低剂量组Scr值升高。结论雷公藤的肝毒性较强,且毒性成分集中在醇提物部位,而以乙酸乙酯部位和正丁醇部位较多。雷公藤不同部位短期给药产生的肾脏毒性不明显,对肾脏产生的影响主要和醇提物部位有关。
Objective To observe the effect of different extraction sites of Tripterygium wilfordii on liver and kidney toxicity in mice. Methods The different parts of Tripterygium wilfordii were extracted and the mice were drenched for 5 and 10 d respectively. The function indexes of liver and kidney injury in mice were detected and the more obvious parts of liver and kidney damage were studied. Results After administration for 5 and 10 days, the ethanol extracts, ethyl acetate and n-butanol fractions of the mice were able to increase the levels of alanine aminotransferase and aspartate aminotransferase in the blood of mice. After 5 days of administration, But the serum creatinine (Scr) had little effect. After 10 days of administration, the n-butanol high-dose group and the n-butanol high-dose group Alcohol extract high and low dose group BUN increased alcohol extract high and low dose group Scr value increased. Conclusion Tripterygium wilfordii hepatotoxicity is strong, and toxic components concentrated in the alcohol extract parts, and parts of ethyl acetate and n-butanol more. Triptolide different parts of short-term renal toxicity caused by the drug is not obvious, the main impact on the kidney and ethanol extract parts.