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目的:建立一种免疫捕捉法PCR技术,研究血清免疫复合物中不同Ig类型抗体结合HBV的情况,并初步探讨其意义。方法:以羊抗人μ、γ、α链的IgG为捕捉抗体,再利用PCR扩增捕捉物中的HBVDNA。结果:成功地建立了检测HBVDNA/IgM、IgG和IgATCIC的免疫捕捉法PCR技术。该技术特异性强、敏感性高、重复性好,可以显著提高HBVDNA的检出率。同时发现,结合HBV的抗体的Ig类型组合有明显差异,三者均阳性最高。结论:说明乙肝患者血清免疫复合物中有较多的病毒颗粒。研究HBVDNA/IgTCIC对乙肝发病机理的深入阐明可能具有重要意义。
OBJECTIVE: To establish an immunocapture PCR technique to study the binding of different Ig-type antibodies in serum immune complexes to HBV and to explore its significance. Methods: IgG antibodies against human μ, γ and α chains were used as capture antibody, and PCR amplification of HBVDNA in capture material was performed. Results: The immunoprecipitation PCR technique for the detection of HBVDNA / IgM, IgG and IgATCIC was successfully established. The technical specificity, high sensitivity, good repeatability, can significantly improve the detection rate of HBVDNA. At the same time, it was found that there was a significant difference in the Ig type combination of the antibodies bound to HBV, all of which were the highest positive. Conclusion: There are more virus particles in the serum immune complexes of hepatitis B patients. The study of HBVDNA / IgTCIC on hepatitis B pathogenesis in-depth elucidation may be of great significance.