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用高效液相色谱法定量分析了７－酮基胆固醇在胎肝细胞上清液及细胞裂解液中的含量。色谱柱为μ－ＰｏｒａｓｉｌＳｉＯ２柱，流动相为正己烷∶异丙醇（９１∶９，Ｖ／Ｖ）。方法回收率高、色谱重复性好、分析速度快
The content of 7-keto-cholesterol in fetal liver cell supernatant and cell lysate was quantitatively analyzed by high-performance liquid chromatography. The column was μ-PorasilSiO2 column and the mobile phase was n-hexane: isopropanol (91: 9, V / V). The method has high recovery rate, good chromatographic repeatability and fast analysis speed