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目的：探讨肿瘤转移抑制基因ｎｍ２３－Ｈ１表达产物ｎｍ２３－Ｈ１ｍＲＮＡ在胰腺癌组织中的表达及其与预后的关系。方法：应用ＲＴ－ＰＣＲ及Ｓｏｕｔｈｅｒｎｂｌｏｔ方法，检测２７例胰腺癌及７例正常胰腺组织中ｎｍ２３－Ｈ１ｍＲＮＡ的表达。结果：７例正常胰腺组织仅１例表达ｎｍ２３－Ｈ１ｍＲＮＡ，占１４．２８％，２７例胰腺癌标本中有１９例表达ｎｍ２３－Ｈ１ｍＲＮＡ，占７０．２５％。经卡方检验发现：ｎｍ２３－Ｈ１ｍＲＮＡ表达与胰腺癌病理分级、临床分期及伴随局部淋巴结转移呈显著相关（Ｐ＜０．０５）。结论：检测ｎｍ２３－Ｈ１ｍＲＮＡ表达对判断胰腺癌恶性程度及预测预后有重要参考价值。
Objective: To investigate the expression of nm23-H1 mRNA, a metastasis suppressor gene, in pancreatic cancer and its relationship with prognosis. Methods: The expression of nm23-H1 mRNA in 27 cases of pancreatic cancer and 7 cases of normal pancreas was detected by RT-PCR and Southern blot. Results: In 7 cases of normal pancreatic tissue, only one case expressed nm23-H1 mRNA, accounting for 14.28%. Among 27 cases of pancreatic cancer, 19 cases expressed nm23-H1 mRNA, accounting for 70.25%. After chi-square test, it was found that the expression of nm23-H1 mRNA was significantly correlated with pathological grade, clinical stage and regional lymph node metastasis of pancreatic cancer (P<0.05). Conclusion: The detection of nm23-H1 mRNA expression has important reference value in judging the malignant degree of pancreatic cancer and predicting the prognosis.