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目的:研究蛋白酶体抑制剂MG132与顺铂(DDP)联合应用对人鼻咽癌细胞株CNE-1细胞凋亡的影响,观察细胞中凋亡相关蛋白Bcl-2和Bad表达的变化。方法:体外培养的CNE-1细胞分别暴露于MG132和DDP、单独MG132或者DDP,24h后,流式细胞术(FCW)检测CNE-1细胞的凋亡率,蛋白质印迹法测定CNE-1细胞中Bcl-2和Bad蛋白的表达情况。结果:流式细胞术检测结果显示,MG132和DDP联合用药组CNE-1细胞凋亡率(53±3.2)%较单独应用MG132组(20±1.7)%、DDP组(18±2.3)%的凋亡率显著增加。蛋白质检测结果显示,与MG132组、DDP组比MG132和DDP组Bcl-2的表达减少约3倍,而Bad的表达增加约2倍。结论:MG132和DDP联合应用可能通过降低Bcl-2同时增强Bad的表达而进一步诱导CNE-1细胞的凋亡。因此,我们可以认为MG132能够增强DDP对CNE-1细胞凋亡的作用。
AIM: To investigate the effect of proteasome inhibitor MG132 and cisplatin (DDP) on the apoptosis of human nasopharyngeal carcinoma cell line CNE-1 and to observe the changes of apoptosis-related proteins Bcl-2 and Bad. Methods: The CNE-1 cells cultured in vitro were exposed to MG132 and DDP, MG132 or DDP alone, respectively. The apoptosis rate of CNE-1 cells was detected by flow cytometry (FCW) and the expression of CNE- Bcl-2 and Bad protein expression. Results: The results of flow cytometry showed that the apoptosis rate of CNE-1 cells in MG132 and DDP group was (53 ± 3.2)% compared with that in MG132 group (20 ± 1.7%) and that of DDP group (18 ± 2.3)% Apoptosis rate increased significantly. The result of protein test showed that compared with MG132 group, the expression of Bcl-2 in DDP group decreased about 3 times and that of Bad increased about 2 times compared with MG132 and DDP group. Conclusion: The combination of MG132 and DDP may further induce the apoptosis of CNE-1 cells by decreasing the expression of Bcl-2 and increasing the expression of Bad. Therefore, we can consider that MG132 can enhance the effect of DDP on CNE-1 cell apoptosis.