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[Objective] This study aimed to investigate the effects of 5-aminolevulinic acid( ALA) on the content of total flavonoids and relative expression levels of chalcone sythase( CHS) and chalcone isomerase( CHI) genes in young apples to determine the appropriate ALA concentration and processing time. [Method]Before thinning,young apples were treated with 0( CK),100,200,300 and 400 mg / L ALA. At 12 d after ALA treatment,the content of total flavonoids in young apples was determined by ultraviolet spectrophotometry. The relative expression levels of CHS and CHI genes in young apples were determined by q PCR. [Result]When ALA concentration was 0- 300 mg / L,the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples were improved with the increase of ALA concentration. As ALA concentration rose to 400 mg / L,various indicators showed a downward trend. Moreover,the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples treated with different concentrations of ALA were improved significantly,which reached the maximum at 9 d and declined since 12 d. [Conclusion] Compared with CK,spraying young apples with 300 mg / L ALA at 6- 9 d before thinning was conducive to improving the content of flavonoids in thinned young apples.
[Objective] This study aimed to investigate the effects of 5-aminolevulinic acid (ALA) on the content of total flavonoids and relative expression levels of chalcone sythase (CHS) and chalcone isomerase (CHI) genes in young apples to determine the appropriate ALA concentration At 12 days after ALA treatment, the content of total flavonoids in young apples was determined by ultraviolet spectrophotometry. The relative expression levels of CHS and CHI genes in young apples were determined by q PCR. [Result] When ALA concentration was 0-300 mg / L, the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples were improved with the increase of ALA concentration. As ALA concentration rose to 400 mg / L, various indicators showed a downward trend. Moreover, the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples treated with different concentrations of ALA were significantly increased, which reached the maximum at 9 d and canceled since 12 d. [Conclusion] Compared with CK, spraying young apples with 300 mg / L ALA at 6- 9 d before thinning was conducive to improving the content of flavonoids in thinned young apples.