CRYSTALLOGRAPHIC STUDIES OF THE COMPLEX OF MUNG BEAN TRYPSIN INHIBITOR WITH PORCINE TRYPSIN

• 作 者: 张荣光 林光大 严友为 唐伟中 戚正武 曹天钦
• 机 构: Shanghai Institute of Biochemistry, Academia Sinicarrrrrrrrn,Shanghai Institute of Biochemi
• 出 处: Science in China,Ser.B
• 发表日期: 1985年09期
• 关键词: tetragonal dimensions molar Bowman porcine trypsin combine asymmetric belongs bo

The Bowman-Birk family inhibitor isolated from the mung bean can combine with porcine trypsin to form a complex at a molar ratio of 1:2. The single crystals of the complex, suitable for X-ray structural study, were obtained by using the micro-still-setting method. Initial crystallographic investigation showed that the crystals diffract to 2.7 resolution and belong to the tetragonal system. Its space group is 1422 with cell dimensions a=b=122.4 (2), c=113.4(2). The density of the crystal was 1.20g/cm~3 and the solvent constant was 36%. There is one complex molecule in a crystallographic asymmetric unit.

The Bowman-Birk family inhibitor isolated from the mung bean can combine with porcine trypsin to form a complex at a molar ratio of 1: 2. The single crystals of the complex, suitable for X-ray structural study, were obtained by using the micro -still-setting method. Initial crystallographic investigation showed that the crystals diffract to 2.7 resolution and belong to the tetragonal system. Its space group is 1422 with cell dimensions a = b = 122.4 (2), c = 113.4 (2) of the crystal was 1.20 g / cm ~ 3 and the solvent constant was 36%. There is one complex molecule in a crystallographic asymmetric unit.