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Vpr是人类免疫缺陷病毒(Human Immunodeficiency Virus type 1,HIV-1)的辅助蛋白之一,在病毒复制及AIDS进程中起重要作用。为了研究Vpr完成其生物学功能的分子机制,本研究利用酵母双杂交技术,从人的cDNA文库中筛选,并经免疫共沉淀技术证实NF-κB通路中的重要蛋白RelB与Vpr存在相互作用;发现RelB蛋白能促进Vpr介导的对NF-κB报告基因的激活,也能促进Vpr对HIV-1LTR的反式激活作用。利用流式细胞技术发现RelB促进Vpr诱导细胞周期G2/M期停滞。上述结果表明,RelB辅助Vpr完成其转录激活以及调控细胞周期的功能。
Vpr is one of the accessory proteins of Human Immunodeficiency Virus type 1 (HIV-1) and plays an important role in virus replication and AIDS progression. In order to study the molecular mechanism by which Vpr completes its biological function, yeast two-hybrid technique was used to screen human cDNA library and confirmed by co-immunoprecipitation that RelB, an important protein in NF-κB pathway, interacts with Vpr. RelB protein was found to promote Vpr-mediated NF-κB reporter gene activation, but also promote the Vpr HIV-1LTR transactivation. Using flow cytometry found that RelB promoted Vpr induced cell cycle G2 / M arrest. The above results indicate that RelB assists Vpr in its transcriptional activation and in the regulation of cell cycle.