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目的以高效液相色谱法测定定心胶囊中有效成分之一丹参酚酸B的含量,以明确其作用机理。方法采用Hypersil ODS(5μ,4.6mm×200mm)色谱柱,甲醇-乙腈-甲酸-水(30:10:1:59)为流动相,检测波长286nm。结果丹参酚酸B在0.1024μg～0.6146μg范围内呈良好的线性关系,r=0.9999;平均回收率为96.66%(n=9),RSD=0.9%。结论所建立的方法稳定、可靠,可为该产品建立质量控制方法提供参考。
OBJECTIVE To determine the content of salvianolic acid B, one of the active ingredients in Dingxin Capsules, by high performance liquid chromatography (HPLC) to clarify the mechanism of action. Methods The mobile phase consisted of Hypersil ODS column (5μ, 4.6 mm × 200 mm), methanol-acetonitrile-formic acid-water (30:10:::59) and the detection wavelength was 286 nm. Results Salvia miltiorrhiza B had a good linear relationship in the range of 0.1024μg ~ 0.6146μg, r = 0.9999; the average recovery was 96.66% (n = 9), RSD = 0.9%. Conclusion The established method is stable and reliable and can provide a reference for the establishment of quality control methods for this product.