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目的:探讨百事乐胶囊对慢性应激抑郁模型大鼠行为活动、海马神经元凋亡及凋亡因子B细胞淋巴瘤/白血病2(Bcl-2),Bcl-2相关x蛋白(Bax)表达的影响。方法:将SD大鼠随机分为6组,即空白对照、抑郁模型、氟西汀、百事乐胶囊高、中、低(2.88,1.44,0.72 g·kg-1)剂量组,采用慢性温和不可预见性应激加孤养的方式建立抑郁模型,造模同时ig给药,连续21d,测定各组大鼠体重变化,Open-field、1%蔗糖偏食度测定大鼠行为学变化,TUNEL染色测定海马神经元凋亡变化,免疫组化检测海马Bcl-2,Bax的表达。结果:与正常组比较,模型组体重增加、水平及垂直活动次数、蔗糖偏食度明显下降(P<0.01),凋亡细胞所占比例及凋亡细胞计数明显增加(P<0.01);Bcl-2表达下降(P<0.01),Bax表达上升(P<0.01)。百事乐胶囊高剂量在第14、21天提升模型大鼠体重(P<0.01),中剂量在第21天升高模型大鼠体重(P<0.05);高、中剂量能提升模型大鼠水平或垂直活动次数(P<0.01或P<0.05);高、中剂量能提高模型大鼠蔗糖偏食度,降低CA1,CA3区凋亡细胞所占比例及凋亡细胞计数(P<0.01或P<0.05),且高剂量能降低模型大鼠DG区凋亡细胞所占比例及凋亡细胞计数,升高海马CA3区Bcl-2并降低Bax的表达(P<0.05)。结论:百事乐胶囊能通过减少海马神经元的凋亡而达到抗抑郁的作用。
Objective: To investigate the effects of Pepsi Capsule on behavioral activity, hippocampal neuronal apoptosis and the expression of Bcl-2 and Bcl-2 in rats with chronic stress depression influences. Methods: SD rats were randomly divided into 6 groups: blank control, depression model, fluoxetine, Pepsi Capsule high, medium and low dose groups (2.88,1.44, 0.72 g · kg -1) The model of depression was established by prophylactic stress and loneliness. At the same time, the rats were given ig administration for 21 days. The body weight of the rats in each group was measured. The open-field, 1% sucrose partial eclipse was used to measure the change of behavior. TUNEL staining The changes of apoptosis in hippocampus neurons were detected by immunohistochemistry. The expressions of Bcl-2 and Bax in hippocampus were detected by immunohistochemistry. Results: Compared with the normal group, body weight, horizontal and vertical activities and sucrose partial eclipse decreased significantly (P <0.01), the percentage of apoptotic cells and apoptotic cells increased significantly (P <0.01) 2 expression decreased (P <0.01), Bax expression increased (P <0.01). The high dose of Pepsi Capsule increased the body weight of model rats (P <0.01) on the 14th and 21st days, the middle dose increased the body weight of rats on the 21th day (P <0.05), and the high and middle doses increased the level of the model rats Or the number of vertical activities (P <0.01 or P <0.05). The high and middle doses could increase the sucrose partial eclipse and reduce the percentage of apoptotic cells and apoptotic cells in CA1 and CA3 area (P <0.01 or P < 0.05), and the high dose could reduce the percentage of apoptotic cells and apoptotic cells in DG area of hippocampus, increase Bcl-2 in hippocampal CA3 area and decrease the expression of Bax (P <0.05). Conclusion: Pepsi Capsule can achieve antidepressant effect by reducing the apoptosis of hippocampal neurons.