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目的了解胃癌组织中端粒酶表达的状况及其临床意义,研究非放性 TRAP 检测端粒酶的可行性。方法用 TRAP 银染法检测58例胃癌及相应患者的正常胃粘膜,12例胃腺瘤和9例胃溃疡组织的端粒酶表达情况。结果 58例胃癌组织中49(84.5％)例端粒酶表达阳性,正常粘膜无一例检测到端粒酶活性(P<0.001),12例胃腺瘤,9例胃溃疡组织中各有1例检测到端粒酶活性。胃癌组织端粒酶的表达与年龄、肿瘤大小、分化程度、浸润深度、淋巴结转移及 TNM 分期等临床病理参数无明显相关(P<0.05)。结论端粒酶是一种较理想的胃癌肿瘤标记物,TRAP 银染法是一种值得推广的检测端粒酶活性的方法。
Objective To understand the status of telomerase expression in gastric cancer tissues and its clinical significance, and to investigate the feasibility of using non-release TRAP to detect telomerase. Methods TRAP silver staining was used to detect the expression of telomerase in 58 cases of gastric cancer and the corresponding normal gastric mucosa, 12 cases of gastric adenoma and 9 cases of gastric ulcer tissue. Results The expression of telomerase was positive in 49 cases (84.5%) in 58 cases of gastric cancer. No telomerase activity was detected in normal mucosa (P<0.001). There were 12 cases of gastric adenoma and 1 case of 9 cases of gastric ulcer. To telomerase activity. The expression of telomerase in gastric cancer tissues was not significantly related to the clinicopathological parameters such as age, tumor size, differentiation, depth of invasion, lymph node metastasis, and TNM stage (P<0.05). Conclusion Telomerase is an ideal tumor marker for gastric cancer. TRAP silver staining is a method worthy of generalizing the detection of telomerase activity.