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作用介绍了柱前固相丹磺酰化测定儿茶酚胺的灵敏和选择性的高效液相色谱方法.肾上腺素(EP),去甲肾上腺素(NE)和多巴胺(DA)这些儿茶酚胺(CAs)的标准贮备液,由溶于0.04M的高氯酸中并稀释到浓度为1μmol/ml,贮藏于4℃,临用前仍用0.04M的高氯酸稀释,内标物3.4-二羟基甲苯胺(DHBA)溶液也用类似的方法配制.儿茶酚胺的测定步骤:将50μl的内标液(1.0nmol/ml)和200μl儿茶酚胺溶液或尿液一起加入含有25mg氧化铝和2mgEDTA-Na_2的试验管中,在加入100μl 3M Tris/HCl缓冲溶液(pH8.6)后,于室温下将试管缓慢地振摇10分钟,氧化铝用30ml水冲洗;然后吸出冲洗液,再加入200μl的丹磺酰
This article introduces the sensitive and selective high performance liquid chromatography (HPLC) method for the determination of catecholamines by precolumn solid-phase dansylization. The standards of catecholamines (CAs) such as epinephrine (EP), norepinephrine (NE) and dopamine (DA) Stock solution, dissolved in perchloric acid 0.04M and diluted to a concentration of 1μmol / ml, stored at 4 ℃, before use is still 0.04M perchloric acid dilution, the internal standard 3.4-dihydroxytoluidine ( DHBA) was also prepared in a similar manner Catecholamine Assay Procedure: 50 μl of an internal standard solution (1.0 nmol / ml) was added to a test tube containing 25 mg of alumina and 2 mg of EDTA-Na 2 together with 200 μl of catecholamine solution or urine, After adding 100 μl of 3M Tris / HCl buffer solution (pH 8.6), the tube was gently shaken at room temperature for 10 minutes and the alumina was rinsed with 30 ml of water; then the rinse solution was aspirated and 200 μl of dansyl