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目的：观察血小板活化因子（ＰＡＦ）拮抗剂对急性出血坏死型胰腺炎（ＡＨＮＰ）大鼠内毒素血症的防治作用。方法：１４０只ＳＤ大鼠随机分为３组：急性胰腺炎组（ＡＰ组）：采用去氧胆酸钠逆行胰管内注射法复制；治疗组（ＢＮ组）：制备模型后经腹腔注射ＰＡＦ特异性受体拮抗剂ＢＮ５２０２１（５ｍｇ／ｋｇ）；假手术组（ＳＯ组）：开腹后仅轻轻翻动胰腺即关腹。结果：ＢＮ组与ＡＰ组比较，１小时后血清淀粉酶值明显下降〔（１４９７０±２５００）Ｕ／Ｌ，（１６１７０±２３８０）Ｕ／Ｌ，Ｐ＜０．０５〕，６小时和１２小时后更为明显（Ｐ均＜０．０１）；血中ＰＡＦ含量１小时后明显降低〔（２．２０±０．２５）μｇ／Ｌ与（１．１０±０．２１）μｇ／Ｌ，Ｐ＜０．０５〕，３小时后更为明显（Ｐ＜０．０１）。血浆内毒素含量ＢＮ组比ＡＰ组明显下降（Ｐ＜０．０１）。ＢＮ组术后大鼠平均存活时间为（４５．０±２５．１）小时，存活率为４０％；ＡＰ组术后大鼠在２４小时内全部死亡，平均存活时间为（１１．５±４．８）小时，存活率为０（Ｐ均＜０．０１）。结论：ＰＡＦ参与了ＡＨＮＰ的发病过程；应用ＰＡＦ受体拮抗剂对实验性ＡＨＮＰ有良好的防治作用。
Objective: To observe the preventive and therapeutic effects of platelet activating factor (PAF) antagonists on endotoxemia in acute hemorrhagic necrotizing pancreatitis (AHNP) rats. Methods: One hundred and forty SD rats were randomly divided into three groups: Acute pancreatitis group (AP): sodium desoxycholate retrograde pancreatic ductal injection. The treatment group (BN group): After the model was prepared, the intraperitoneal injection of PAF Sexual receptor antagonist BN52021 (5mg / kg); sham group (SO group): Open the pan only gently flip the pancreas is closed. Results: Compared with AP group, serum amylase level in BN group and AP group was significantly decreased (14970 ± 2500 U / L, 16170 ± 2380 U / L, P <0.05), and after 6 hours and 12 hours (P <0.01). The levels of PAF in blood were significantly lower at 1 hour (2.20 ± 0.25 μg / L vs 1.10 ± 0.21 μg / L, P < 0.05〕, more obvious after 3 hours (P <0.01). Plasma endotoxin levels in BN group were significantly lower than those in AP group (P <0.01). In the BN group, the average survival time was (45.0 ± 25.1) hours and the survival rate was 40% in the rats after operation. The rats in the AP group died within 24 hours and the average survival time was 11.5 ± 4 .8) hours, the survival rate was 0 (P <0.01). Conclusion: PAF is involved in the pathogenesis of AHNP. PAF receptor antagonist has a good preventive and therapeutic effect on experimental AHNP.