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以低芥酸油菜品系APL01与高芥酸品种M083杂交获得的6个基本世代(P1、P2、F1、B1、B2和F2)为材料,利用主基因+多基因混合遗传模型对油菜脂肪酸组成中的芥酸含量进行遗传分析。结果表明:芥酸含量由2对加性-显性主基因控制,2对主基因的加性效应值分别为-12.27和-8.83,显性效应值分别为0.35和1.69,加性效应显著大于显性效应;芥酸含量的主基因遗传率较高,为92.54%~96.72%。以(APL01×M083)BC1F1为作图群体,利用251个分子标记,构建了由19个连锁群组成的分子标记遗传图谱,并利用W inQTLCart 2.0对种子芥酸含量进行QTL扫描,获得2个与芥酸含量相关的QTL,其中qER8位于N8连锁群的m11 e37b~A0226Ba267区间,效应值为-8.32,可解释芥酸含量表型变异为16.74%;qER13位于N13连锁群的A0301Bb398~m18 e46区间,效应值为-9.12,可解释芥酸含量表型变异为31.32%。qER8和qER13两侧分子标记m11 e37b、A0226Ba267、A0301Bb398和m18 e46可用于芥酸含量的标记辅助选择。
Six basic generations (P1, P2, F1, B1, B2 and F2) obtained from the cross between low erucic acid line APL01 and high erucic acid variety M083 were used as materials. The main gene plus polygene mixed genetic model Erucic acid content for genetic analysis. The results showed that erucic acid content was controlled by two pairs of additive-dominance major genes. The additive effects of two major genes were -12.27 and -8.83, respectively. The dominant effects were 0.35 and 1.69, respectively. The additive effects were significantly greater than Dominant effect; eucalyptus content of the main gene heredity rate was 92.54% ~ 96.72%. Using BC1F1 (APL01 × M083) as a mapping population, 251 molecular markers were used to construct a molecular marker genetic map composed of 19 linkage groups. QTLs of erucic acid content in seeds were screened using W inQTLCart 2.0 to obtain 2 QTL related to erucic acid content, of which qER8 located in the N8 linkage group m11 e37b ~ A0226Ba267 interval, the effect value was -8.32, to explain erucic acid content of phenotypic variation was 16.74%; qER13 located in N13 linkage group A0301Bb398 ~ m18 e46 interval , The effect value of -9.12, erucic acid content can explain the phenotypic variation of 31.32%. The markers m11 e37b, A0226Ba267, A0301Bb398 and m18 e46 on both sides of qER8 and qER13 can be used as marker-assisted selection for erucic acid content.