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本文报道了马尾松毛虫质型多角体病毒（简称ＤｐＣＰＶ）在５株昆虫细胞系，即棉铃虫卵巢细胞系ＳＦＥ－ＨＡ－８３１与ＳＦＥ－ＨＡ－８２１２、家蚕细胞系Ｂｍ－Ｎ、粉纹夜蛾细胞系ＴＮ－３６８和草地贪夜蛾细胞系Ｓｆ－２１中的增殖。证明ＤｐＣＰＶ可在ＳＦＥ－ＨＡ－８３１、ＳＦＥ－ＨＡ－８２１２、ＴＮ－３６８和Ｓｆ－２１４株细胞系中增殖。其中ＳＦＦ－ＨＡ－８３１细胞能同时复制几种型混合的ＤｐＣＰＶ；细胞的感染率在２０％－３５％左右，每个感染细胞约可产生５０－１００个多角体；感染细胞己传代２０次以上；离体和活体增殖的病毒的形态、感染力和核酸带谱等特征均保持稳定。
In this paper, three strains of insect cell lines of Dendrolimus punctatus (DpCPV), SFE-HA-831, SFE-HA-8212, Bm-N, Proliferation in the moth cell line TN-368 and the Spodoptera frugiperda cell line Sf-21. It was demonstrated that DpCPV can proliferate in SFE-HA-831, SFE-HA-8212, TN-368 and Sf-214 strain cell lines. Among them, SFF-HA-831 cells can replicate several types of mixed DpCPV simultaneously; the infection rate of cells is about 20% -35%, and about 50-100 polyhedra are produced per infected cell; the infected cells have been passaged more than 20 times In vitro and in vivo proliferation of virus morphology, infectivity and nucleic acid bands and other characteristics remain stable.