论文部分内容阅读
用５×１０（－３）ｍｏｌ／Ｌ环六亚甲基双乙酰胺和３×１０（－６）ｍｏｌ／Ｌ亚硒酸钠诱导剂组合处理人胃腺癌ＭＧｃ８０－３细胞，结果细胞的群体增殖和ＤＮＡ合成均受到显著抑制，生长抑制率最高达７２．８％，ＤＮＡ合成的抑制率达８４．１％，液门测定的ＣＰＭ值也较诱导处理之前降低了５．２６倍；同时，ＭＧＣ８０－３细胞的表型由典型恶性特征向相应正常细胞类似的形态结构特征转变，细胞对裸鼠的致瘤性也明显降低（Ｐ值＜０．０５）·表明ＨＭＢＡ和亚硒酸钠的组合能够有效地诱导人胃腺癌细胞的分化，而且这种诱导分化作用比单独使用其中一种诱导物所产生的效果更显著．
Treatment of human gastric adenocarcinoma MGc80-3 cells with a combination of 5×10 (-3) mol/L cyclohexamethylene bisacetamide and 3×10 (-6) mol/L sodium selenite inducer, resulting in a population of cells Proliferation and DNA synthesis were both significantly inhibited, the growth inhibition rate was up to 72.8%, the inhibition rate of DNA synthesis was 84.1%, and the CPM value measured by the liquid gate was also reduced by 5.26 times before the induction treatment; The phenotype of MGC80-3 cells changed from a typical malignant feature to a similar morphological feature of the corresponding normal cells, and the tumorigenicity of the cells to nude mice was also significantly reduced (P<0.05). This indicates that HMBA and sodium selenite The combination can effectively induce the differentiation of human gastric adenocarcinoma cells, and this effect of inducing differentiation is more significant than that of using one of the inducers alone.