论文部分内容阅读
目的:评价市售阿霉素脂质体体外逆转肿瘤多药耐药(MDR)的作用。方法:MTT法比较阿霉素和阿霉素脂质体体外对敏感和耐药细胞的细胞毒作用;荧光显微镜观察2组药物对敏感和耐药细胞的影响;流式细胞仪检测2组分别在细胞内的积累和外排情况。结果:细胞毒性试验结果显示,尽管在敏感细胞中,阿霉素脂质体IC50远高于阿霉素溶液组,对于耐药细胞株,阿霉素脂质体的IC50与游离阿霉素无显著性差异(P>0.05);细胞荧光染色显示,阿霉素脂质体较溶液在耐药细胞核中有更强的红染;细胞摄取试验显示,相同浓度下,阿霉素脂质体较溶液组在耐药细胞中积累量差异无显著性(P>0.05),但外排试验显示,在耐药细胞KBv200中,相同浓度下的脂质体较溶液具有更强的细胞滞留能力(P<0.05)。结论:阿霉素脂质体较阿霉素溶液在体外能更多进入耐药细胞核,并表现出更强的药物滞留能力,可部分克服多药耐药。
PURPOSE: To evaluate the role of commercial doxorubicin liposomes in reversing multidrug resistance (MDR) in vitro. Methods: MTT assay was used to compare the cytotoxic effect of doxorubicin and doxorubicin liposomes on sensitive and resistant cells in vitro. Fluorescence microscopy was used to observe the effect of two drugs on the sensitive and resistant cells. Flow cytometry Accumulation and efflux in the cells. RESULTS: The results of cytotoxicity assay showed that IC50 of doxorubicin liposomes was significantly higher than that of adriamycin-free liposomes (P> 0.05). Fluorescent staining showed that doxorubicin liposomes had stronger red staining than drug solution in the drug-resistant nuclei. Cell uptake assay showed that at the same concentration of doxorubicin liposomes (P> 0.05). However, the efflux assay showed that the liposomes at the same concentration in the drug-resistant cell line KBv200 had stronger cell retention capacity than the solution (P <0.05). CONCLUSION: Doxorubicin liposomes can penetrate into drug-resistant nucleus more than doxorubicin solution in vitro, and show more drug retention ability, which can partially overcome multidrug resistance.