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近年来生物发光(BL)和化学发光(CL)反应已成为临床实验室的核心技术之一。在CL 反应中,弱键分解时释放的化学能产生了激发态的中间物,后者衰变成基态时就发射出光。BL 是自然界中一种特殊类型的CL,酶或发光蛋白可促进发光。CL 和BL 最显著的进展发生于下列领域应用中,即免疫分析、DNA 杂交分析、印渍分析和DNA 序列分析.一、优点和缺点灵敏度一般CL 和BL 分析的检测限度在亚pmol 水平(如血清胆汁酸BL 检测限度为0.5pmol),均超过了荧光分析和比色分析的灵敏度。但CL 和
In recent years, bioluminescence (BL) and chemiluminescence (CL) reactions have become one of the core technologies in clinical laboratories. In the CL reaction, the chemical energy released during the weak bond dissociation produces an intermediate in the excited state, which emits light when decayed to the ground state. BL is a special type of CL in nature and enzymes or photoproteins promote luminescence. The most significant advances in CL and BL have occurred in applications such as immunoassays, DNA hybridization analysis, blot analysis, and DNA sequence analysis. Advantages and Disadvantages Sensitivity The limits of detection for CL and BL analysis are generally at sub-pmol levels Serum bile acid BL detection limit of 0.5pmol), have exceeded the fluorescence analysis and colorimetric sensitivity. But CL and