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目的研究链霉菌183发酵液中抗乙型肝炎病毒活性成分。方法通过超滤法和凝胶过滤层析进行分离纯化,用高效凝胶渗透法和多角度激光光散射法测定分子量,用酸水解、糖醇乙酸酯衍生化和气相色谱测定单糖组成和摩尔比,用鸭乙肝病毒复制复合体DNA聚合酶/逆转录酶筛选模型,2.2.15细胞和鸭乙型肝炎病毒动物模型评价抗乙肝病毒活性。结果分离得到183A、B、C、D四个多糖纯品,183B在2.2.15细胞内抑制HBV DNA的IC50为2.62μg/mL,粗多糖在接近鸭最大耐受剂量时对鸭乙肝病毒显示一定的抑制作用。结论在酶水平和细胞水平上的两个不同层次的模型中,183各多糖组分的抗病毒效果并不平行,183粗多糖对病毒复制关键酶及2.2.15细胞内HBV复制的抑制作用较强。
Objective To study the anti-HBV activity of Streptomyces 183 fermentation broth. Methods The compounds were isolated and purified by ultrafiltration and gel filtration chromatography. The molecular weight was determined by high performance gel permeation and multi-angle laser light scattering. The content of monosaccharide and the content of monosaccharide were determined by acid hydrolysis, sugar alcohol acetate derivatization and gas chromatography Molar ratio, using duck hepatitis B virus replication complex DNA polymerase / reverse transcriptase screening model, 2.2.15 cells and duck hepatitis B virus animal model to evaluate anti-hepatitis B virus activity. Results The purified polysaccharides of 183A, B, C and D were obtained. The IC50 of 183B in 2.2.15 cells inhibiting HBV DNA was 2.62μg / mL. The crude polysaccharide showed a certain level of duck hepatitis B virus at the maximum tolerance dose of duck Inhibition. Conclusions In the two levels of enzyme level and cellular level model, the antiviral effect of 183 polysaccharide components is not parallel, 183 crude polysaccharide on viral replication key enzyme and 2.2.15 intracellular HBV replication inhibition than Strong.