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水分利用效率(water use efficiency,WUE)育种有助于通过节水灌溉缓解用水短缺的问题.但是WUE是一个复杂的数量性状,许多研究者并不直接地检测这一指标.本研究通过精细定量水分供应实验,利用由1种籼稻型水稻材料和1种粳稻型旱稻材料杂交后产生的重组自交系,探寻水稻WUE的遗传机理.对抽穗期(heading date)、单日耗水量(water/d)、地上部干重单日累积量(shootw/d)、根重单日累积量(rootw/d)、籽粒重单日累积量(kernelw/d)、整株单日水分利用率(WUEwhole/d)、地上部干重单日水分利用率(WUEup/d)、产量单日水分利用率(WUyield/d)、单日经济系数(econindex/d)和单日根冠比(ratio/d)等11个性状进行了统计学分析和QTL定位.结果表明,大部分性状极显著相关.利用复合区间作图法检测出与econindex,econindex/d,WUEyield,WUEyield/d,WUEup,WUEup/d,WUEwhole,WUEwhole/d,kernelw,kernelw/d,rootw和water/d等12个性状相关的24个QTL,分布在第1,2,4,6,7,8和12染色体上.每个QTL解释了54.97%~10.78%的表型变异.这些结果为水稻WUE的分子标记辅助选择和遗传基础的进一步解析提供了有价值的信息.
Water use efficiency (WUE) breeding helps to alleviate water shortages through water-saving irrigation, but WUE is a complex quantitative trait, and many researchers do not directly detect this indicator.By quantitative The water supply experiment was conducted to investigate the genetic mechanism of rice WUE by using recombinant inbred lines derived from a cross of one indica rice material and one japonica type of upland rice.The heading date, d, shootw / d, rootw / d, kernelw / d, WUEwhole / d), WUEup / d, WUyield / d, econindex / d and ratio / d ) And other 11 traits were statistically analyzed and QTL mapping was performed.The results showed that most of the traits were significantly correlated.The econindex, econindex / d, WUEyield, WUEyield / d, WUEup, WUEup / d , 24 QTLs related to 12 traits, such as WUEwhole, WUEwhole / d, kernelw, kernelw / d, rootw and water / d , Distributed on chromosomes 1, 2, 4, 6, 7, 8 and 12. Each QTL explained 54.97% -10.78% of phenotypic variation. These results provide molecular marker-assisted selection and genetic basis for WUE Parsing provides valuable information.