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目的建立东方田鼠免疫抑制模型,初步探讨淋巴细胞在东方田鼠抗血吸虫病机制中的作用。方法成年健康的东方田鼠40只,分成4组(每组10只):免疫抑制+血吸虫感染组、免疫抑制组、血吸虫感染组和空白对照组。免疫抑制剂每周注射3次,连续使用6周,建立东方田鼠免疫抑制的动物模型,用脾淋巴细胞转化试验和ELISA抗体检测进行免疫抑制模型的初步鉴定。在尾蚴攻击感染各组动物后42d,麻醉处死各组动物后进行解剖,灌注冲虫,收集虫体和肝脏虫卵镜检。结果免疫抑制模型淋巴细胞增殖受到显著抑制;免疫抑制组血吸虫抗体水平显著低于未抑制组和空白对照组;免疫抑制和未抑制的东方田鼠均未检出成虫和虫卵。结论成功建立东方田鼠免疫抑制模型,淋巴细胞在东方田鼠抗血吸虫病机制中可能不发挥直接作用。
Objective To establish a model of immunosuppression in Microtus fortis and to explore the role of lymphocytes in the anti-schistosomiasis mechanism of Microtus fortis. Methods 40 adult Microtus Fortis were divided into 4 groups (10 in each group): immunosuppressive + schistosomiasis group, immunosuppressive group, schistosomiasis group and blank control group. Immunosuppressive agents were injected three times a week for 6 weeks continuously to establish an immunosuppressive animal model of Microtus Fortis. Initial identification of the immunosuppressive model was performed using spleen lymphocyte transformation assay and ELISA antibody assay. At 42 days after infection with cercariae, the animals in each group were anesthetized, dissected and perfused with insects to collect the microspheres of the worms and liver. Results The immunosuppressive model of lymphocyte proliferation was significantly inhibited; immunosuppressed group of schistosome antibody levels were significantly lower than the untreated group and the blank control group; immunosuppressed and uninhibited voles were not detected adult voles and eggs. Conclusion The model of immunosuppression in Microtus fortis was successfully established. Lymphocytes may not play a direct role in the anti-schistosomiasis mechanism of Microtus fortis.