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目的:建立HPLC法定量分析罗布麻叶类(红麻和白麻)药材中3种黄酮苷类成分。方法:采用Elite-ODS(5μm,150 mm×4.6 mm)色谱柱,以乙腈-水-四氢呋喃-冰醋酸(8∶81∶10∶1)为流动相,流速1.0 mL.min-1,检测波长258 nm,柱温30℃。结果:芦丁、异槲皮苷和槲皮苷线性范围分别为0.026～0.520,0.094～1.880,0.084～1.680μg;相关系数分别为0.9996,0.9997,0.9990。回收率(n=5)分别为97.5%,98.4%,97.7%;RSD分别为1.9%,1.8%,2.2%。药材红麻和白麻中黄酮苷类成分含量和分布存在差异。结论:所建立的方法快捷、灵敏、准确,可作为鉴别和评价罗布麻叶类药材的质量控制方法。
Objective: To establish a HPLC method for quantitative analysis of three flavonoid glycosides in Apocynaceae (kenaf and white hemp). Methods: The mobile phase was acetonitrile-water-tetrahydrofuran-glacial acetic acid (8:81:10:1) with a flow rate of 1.0 mL · min-1 on a 5 μm, 150 mm × 4.6 mm column. The detection wavelength 258 nm, column temperature 30 ℃. Results: The linear ranges of rutin, isoquercitrin and quercitrin were 0.026-0.520, 0.094-1.880 and 0.084-1.680μg, respectively. The correlation coefficients were 0.9996, 0.9997 and 0.9990 respectively. The recoveries (n = 5) were 97.5%, 98.4% and 97.7%, respectively. The RSDs were 1.9%, 1.8% and 2.2%, respectively. There are differences in the content and distribution of flavonoid glycosides in kenaf and kenaf. Conclusion: The established method is fast, sensitive and accurate and can be used as a quality control method for identification and evaluation of Apocynaceae medicinal materials.