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作者研究了紫外光对苎麻疫霉的抗甲霜灵诱变效应及对苎麻疫霉生物学特性的影响。结果表明,供试6个苎麻疫霉野生型菌株经菌丝块紫外光药剂诱变和菌丝块药剂驯化3周后,均获得抗甲霜灵突变体,且紫外光药剂诱变处理角变区出现频率明显高于药剂驯化处理,说明紫外光对苎麻疫霉抗甲霜灵突变有一定促进效应。紫外光显著地抑制苎麻疫霉游动孢子的萌发和菌丝的生长,在一定范围内,处理时间愈长,抑制率愈高。苎麻疫霉耐紫外光菌株(经亚致死剂量的紫外光照射处理后存活的游动孢子所形成的菌株)与野生型亲本相比,对温度和pH的敏感性大致相同,但菌落形态有一定变异,菌丝生长速率、卵孢子产生量均显著下降,表明紫外光对苎麻疫霉的菌丝生长和卵孢子产生量有明显的抑制作用。苎麻疫霉耐紫外光菌株EC50值比野生型亲本菌株EC50值提高了23.21%-56.70%,即耐紫外光菌株对甲霜灵敏感性比野生亲本菌株显著下降,这与紫外光诱变试验结果是相一致的。
The authors studied the mutagenic effects of UV-light on metalaxyl-resistant metalaxyl and the biological characteristics of Phytophthora ramie. The results showed that mutants of metalaxyl resistant mutant were obtained after mutagenization of mycoplasma variegates and domestication of mycelium in 6 wild ramie genotypes for 3 weeks. The frequency of occurrence in the area was significantly higher than that of the domestication of the chemicals, indicating that ultraviolet light has a certain promoting effect on the resistance of Phytophthora ramets to Metalaxyl. UV light significantly inhibited the germination of Podospora nidulans and mycelium growth, within a certain range, the longer the treatment time, the higher the inhibition rate. Phytophthora ramosensitive UV-resistant strains (strains formed by zoospores that survived sublethal doses of UV light) showed approximately the same sensitivity to temperature and pH compared to the wild-type parent but had some colony morphology Variation, mycelium growth rate, oospores production were significantly decreased, indicating that ultraviolet light on the mycelial growth of ramie and mycoplasma ova significantly inhibited. The EC50 value of Phylum ramosum UV-resistant strains increased by 23.21% -56.70% compared with that of wild-type parental strains, that is, the sensitivity of UV-resistant strains to metalaxyl was significantly lower than that of wild-type parental strains, which was consistent with the UV mutagenicity test Consistent.