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目的 :观察 Td T及系列相关抗原双标记在急性白血病细胞中的表达及其在预测白血病复发过程中的作用。方法 :采用免疫荧光双标记技术 ,配合流式细胞术检测急性白血病细胞的 Td T及系列相关抗原双标记及急性白血病完全缓解后免疫双标记抗原变化。结果 :64例急性白血病中免疫双标记表达 36例 ( 56.2 5% ) ,其中 32例 AL L全部表达免疫双标记 ,32例 AML 仅有 4例表达( 12 .5% ) ,在 12例获 CR的表达免疫双标记的急性白血病中 ,4例免疫双标记细胞占单个核细胞0 .0 1%～ 0 .0 8% ,并有长期缓解 ,而 8例免疫双标记细胞占单个核细胞 1.5%～ 95.38%的急性白血病 ,均于近期骨髓复发。结论 :免疫荧光双标记技术在部分急性白血病的微小残留监测中具有一定的临床意义。
Objective: To observe the expression of Td T and series of related antigen double markers in acute leukemia cells and its role in the prediction of relapse of leukemia. METHODS: Immunofluorescence double-labeling technique was used in combination with flow cytometry to detect Td T and serially-associated antigen double markers in acute leukemia cells and immune double-labeled antigen changes after complete remission of acute leukemia. RESULTS: Among the 64 acute leukemia patients, 36 were immuno-labeled (56.2 5%), of which 32 AL L all expressed double immunolabels, 32 were AML only 4 (12.5%), and 12 were CR. In the double-marker-expressing acute leukemias, 4 cases of immune double-labeled cells accounted for 0.1% to 0.8% of mononuclear cells with long-term remission, and 8 cases of double-labeled cells accounted for 1.5% of mononuclear cells. ~ 95.38% of acute leukemias have recurred in the recent bone marrow. Conclusion : The immunofluorescence double labeling technique has certain clinical significance in the detection of small residuals in some acute leukemia patients.