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目的探究葡萄籽提取物(GSE)对高糖诱导系膜细胞肥大的抑制作用。方法将大鼠系细胞膜分为常规组(NG组)、高糖组(HG组)和高糖联合GSE(高、中、低)组,对蛋白/细胞数量比值、细胞增殖、细胞周期变化和P21与P27表达情况进行分析。结果将NG组为比较对象,其他组在系膜通过高糖连续性培养2 d后,蛋白/细胞数呈现出上升趋势,HG联合中剂量GSE组和HG联合大剂量组的细胞蛋白/细胞数比值呈现出下降趋势,差异有统计学意义(P<0.05)。将NG组为比较对象,其他组经高糖培养48、72 h后,系膜细胞的增值水平减弱,HG联合中剂量GSE组和HG联合大剂量组明显高于HG组,差异有统计学意义(P<0.05)。系膜细胞经过D-葡糖连续刺激2 d后,两种物质的蛋白质水平显著提升,差异有统计学意义(P<0.05)。经高糖联合GSE处理后,以单独高糖组为研究对象,证明GSE可全面对P21和P27起到抑制上升,浓度存在依赖性,差异有统计学意义(P<0.05)。系细胞膜经过高糖连续刺激48 h后,细胞G0/G1期的比例显著上升,和S期相比显著减少,差异有统计学意义(P<0.05)。HG联合中量GSE组和HG联合高粱GSE组细胞的G0/G1比例下降,S期细胞明显提升,差异有统计学意义(P<0.05)。结论 GSE可全面抑制高糖诱导系细胞膜肥大,证明该物质能够全面缓解DN的疾病进展。
Objective To investigate the inhibitory effect of grape seed extract (GSE) on mesangial cell hypertrophy induced by high glucose. Methods The rat plasma membrane was divided into normal group (NG group), high glucose group (HG group) and high glucose combined with GSE (high, medium and low) groups. The protein / cell ratio, cell proliferation, P21 and P27 expression analysis. Results Compared with NG group, the protein / cell number of other groups showed an upward trend after 2 days of continuous culture with high glucose in other groups. The cell protein / cell number of HG combined with medium dose GSE group and HG combined with high dose group Ratio showed a downward trend, the difference was statistically significant (P <0.05). Compared with NG group, the added value of mesangial cells was weakened in other groups after 48,72 h of high glucose culture. HG combined with medium dose of GSE group and HG combined with high dose group was significantly higher than HG group, the difference was statistically significant (P <0.05). Mesangial cells were continuously stimulated by D-glucose for 2 d, the two substances significantly increased the protein levels, the difference was statistically significant (P <0.05). After high glucose and GSE treatment, high glucose alone group as the research object, GSE can fully inhibit the rise of P21 and P27, the concentration-dependent, the difference was statistically significant (P <0.05). The percentage of cells in G0 / G1 phase increased significantly after 48 h of high glucose stimulation, which was significantly lower than that in S phase (P <0.05). The G0 / G1 ratio of HG combined with middle dose GSE group and HG combined with sorghum GSE group decreased, and the number of S phase cells increased significantly, with statistical significance (P <0.05). Conclusion GSE can completely inhibit the hypertrophy of cell membrane induced by high glucose, demonstrating that this substance can completely alleviate the disease progression of DN.