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目的探讨异基因造血干细胞移植(allo-HSCT)患者不同时间点血清生物标志物水平变化和急性移植物抗宿主病(a GVHD)的关系。方法采用蛋白质芯片技术检测20名接受allo-HSCT患者在移植-7 d和+7、+14、+28、+56、+100 d等6个时间点血清IL-1β、IL-7、IL-8、ST2、v WF的含量;其中发生了a GVHD的10例为a GVHD组,另外10例系选择同期未发生a GVHD的病例作为对照组(非a GVHD组)。结果 a GVHD组与非a GVHD组比较,血清ST2含量(灰度值):移植后+7 d为5 475.77±1 681.21 vs 3 362.06±1 360.19,+56 d为6 249.04±2 535.93 vs 3571.62±945.45(P<0.05);IL-7含量(灰度值):移植后+28 d为3 152.35±746.12 vs 1 905.81±860.52,+56 d为3 140.05±772.05 vs 1 816.16±804.56,+100 d为3 560.24±906.78 vs 1 902.56±697.48(P<0.05);IL-1β含量(灰度值):移植后+56 d为4 154.16±1 358.11 vs 2 894.74±1 126.18(P<0.05);其余各检测时间点这3种血清标志物及IL-8、v WF水平6个检测时间点2组相近(P>0.05)。结论 allo-HSCT后a GVHD患者的血清IL-1β、IL-7、ST2含量高于未发生a GVHD者,该3项指标有助于a GVHD的诊断和干预。
Objective To investigate the relationship between serum biomarker level and acute graft versus host disease (GVHD) at different time points in patients with allo-HSCT. Methods The protein expression levels of IL-1β, IL-7 and IL-6 in 20 patients receiving allo-HSCT at 6 d after transplantation and at +7, +14, +28, +56 and + 8, ST2, and vWF. Of the 10 patients who had a GVHD, they were aGVHD, and the other 10 were those who did not have aGVHD during the same period (non-aGVHD). Results Compared with non-GVHD group, the serum ST2 level (gray value) in a GVHD group was 5 475.77 ± 1 681.21 vs 3 362.06 ± 1 360.19 at +7 days after transplantation, 6 249.04 ± 2 535.93 vs 3571.62 ± 945.45 (P <0.05). The content of IL-7 (gray value) was 3252.35 ± 746.12 vs 1 905.81 ± 860.52 on +28 d after transplantation, 3 560.24 ± 906.78 vs 1 902.56 ± 697.48 (P <0.05). The content of IL-1β (grayscale) was 4 154.16 ± 1 358.11 vs 2 894.74 ± 1 126.18 (P <0.05) on the 56th day after transplantation The three serum markers and IL-8 at each detection time point, vWF levels at 6 test time points in two groups were similar (P> 0.05). Conclusion The serum levels of IL-1β, IL-7 and ST2 in patients with a GVHD after allo-HSCT are higher than those without aGVHD. These three indexes are helpful for the diagnosis and intervention of a GVHD.