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目的:分析C57 TgN(HBVadr2.0)SMMU“3号”品系转基因小鼠的月龄、性别和遗传背景与其血清和肝组织中HB sAg表达模式的关系。方法:采用血清ELISA检测和免疫组织化学的方法检测转基因小鼠血清和肝组织中HBsAg的表达,统计学方法分析转基因小鼠血清和肝组织中HBsAg的表达与小鼠的月龄、性别、遗传背景的相关性。结果:转基因小鼠在出生后1~3个月时,血清中HBsAg的表达较低或甚至不表达,8个月龄左右时最高,此后逐渐降低,但肝组织中HBsAg的表达相对稳定在较高水平,能真实反映转基因小鼠中HBsAg的表达。雄性小鼠血清和肝组织中HBsAg的表达量高于雌性小鼠(P<0.05)。转基因小鼠与C57BL/6品系、DAB品系和129s品系正常小鼠交配后的子代,其血清和肝组织中HBsAg表达量均未发生变化。血清阳性的小鼠中肝组织HBsAg阳性率为94.05%,两者呈正相关关系(r=0.257,P<0.01)。结论:C57 TgN(HBVadr2.0)SMMU“3号”品系转基因小鼠血清和肝组织中HBsAg的表达具有发育调节和性别差异。
OBJECTIVE: To analyze the relationship between the age, sex and genetic background of C57 TgN (HBVadr2.0) SMMU “No. 3” transgenic mice and the expression patterns of HBsAg in their serum and liver tissue. Methods: Serum ELISA and immunohistochemistry were used to detect the expression of HBsAg in the serum and liver tissue of transgenic mice. The expression of HBsAg in serum and liver tissues of transgenic mice was analyzed by statistical methods. The age, sex and genetics The relevance of the background. Results: At 1 to 3 months after birth, the expression of HBsAg in serum was low or even not expressed in the transgenic mice, which was the highest at 8 months of age and then decreased gradually. However, the expression of HBsAg in the liver tissue was relatively stable High level, can truly reflect the HBsAg expression in transgenic mice. The expression of HBsAg in serum and liver of male mice was higher than that of female mice (P <0.05). The HBsAg expression levels in serum and liver tissue of transgenic mice did not change after mating with C57BL / 6, DAB and 129s normal mice. The positive rate of HBsAg in liver tissue of seropositive mice was 94.05%, which showed a positive correlation (r = 0.257, P <0.01). Conclusion: The expression of HBsAg in serum and liver of C57 TgN (HBVadr2.0) SMMU “3” transgenic mice has developmental regulation and sex difference.