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目的:探讨ERα-29位基因多态性与HBV相关原发性肝癌(PHC)易感性的关系。方法:通过聚合酶链反应-限制性片段长度多态性法检测我院100例HBV相关原发性肝癌患者(PHC组)与95例同期健康体检者(对照组)的ERα-29位基因多态性并对两组间各等位基因、基因型频率进行比较。结果:PHC组ERα-29位基因的TT、TC和CC基因型分别有31例(31.00%)、45例(45.00%)和24例(24.00%),T与C等位基因频率分别为53.50%和46.50%,对照组ERα-29位基因的TT、TC和CC型基因型分别有11例(11.58%)、39例(41.05%)和45例(47.37%),T与C等位基因频率分别为32.11%和67.89%,两组间基因型分布、等位基因频率差异均具有统计学意义(P<0.05);T等位基因发生PHC的风险是C等位基因的2.43倍(OR=2.43,95%CI:1.37~4.32)。结论:ERα-29位基因与HBV相关原发性肝癌易感性有关,其中T等位基因可增加发生风险。
Objective: To investigate the relationship between ERα-29 gene polymorphism and the susceptibility to HBV-related primary hepatocellular carcinoma (PHC). Methods: The polymorphisms of ERα-29 gene in 100 cases of HBV-related primary hepatocellular carcinoma (PHC) and 95 cases of healthy controls (control group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Morphology and the two groups of alleles, genotype frequencies were compared. Results: The TT, TC and CC genotypes of ERα-29 gene in PHC group were 31 (31.00%), 45 (45.00%) and 24 (24.00%) respectively, and the frequencies of T and C alleles were 53.50 %, And 46.50%. The TT, TC and CC genotypes of ERα-29 gene in control group were 11 (11.58%), 39 (41.05%) and 45 (47.37% The frequency of genotype distribution and allele frequency were 32.11% and 67.89% respectively (P <0.05). The risk of PHC of T allele was 2.43 times of that of C allele = 2.43, 95% CI: 1.37 ~ 4.32). Conclusion: ERα-29 gene is associated with HBV-related susceptibility to primary liver cancer, of which T allele may increase the risk.