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目的:观察腰腿理痛散对大鼠自体髓核移植硬膜外背根节(DRG)中降钙素基因相关肽(CGRP)的影响。方法:取健康Wistar大鼠50只,随机分为假手术组(A组)、模型组(B组)、对照组(C组、腰痛宁组)、高剂量组(D组、腰腿理痛散高剂量组)、低剂量组(E组、腰腿理痛散低剂量组),每组10只,将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧,造成非压迫性大鼠自体髓核移植硬膜外模型,通过免疫组化的方法测定大鼠2周时DRG中CGRP的变化。结果:B、C、D、E组DRG中CGRP阳性神经元的表达明显增加,B、C、D、E组CGRP阳性神经元的免疫反应强度与A组比较均增高(P<0.05),其中B组与A组比较显著增高(P<0.01),但C、D、E组与B组比较为低(P<0.05),D、E组与C组比较为低(P<0.05)。结论:腰腿理痛散可通过下调或者抑制非机械压迫性髓核对神经根损伤后DRG内的痛觉神经递质CGRP的释放而发挥镇痛作用。
Objective: To observe the effect of Yaotong Litong Powder on calcitonin gene-related peptide (CGRP) in rat epidural dorsal root ganglion (DRG) transplanted with autologous nucleus pulposus. Methods: Fifty healthy Wistar rats were randomly divided into sham operation group (A group), model group (B group), control group (C group, Yaotongning group), high-dose group (D group, lower back and leg pain In the high-dose group) and low-dose group (E group, low-dose group in the low-risk and low-dose group), 10 rats in each group took out the rat’s caudal nucleus pulposus and transplanted it into the left epidural L5 and L6 nerves. On the dorsal root side, the epidural model of autologous nucleus pulposus was transplanted in non-stressed rats, and the change of CGRP in DRG was determined by immunohistochemistry. RESULTS: The expression of CGRP-positive neurons in DRG of group B, C, D, and E was significantly increased, and the immunoreactivity of CGRP-positive neurons in group B, C, D, and E was significantly higher than that of group A (P<0.05). The B group was significantly higher than the A group (P<0.01), but the C, D, E group was lower than the B group (P<0.05), and the D, E group was lower than the C group (P<0.05). Conclusion: The treatment of lumbar and leg muscle can exert analgesic effect by down-regulating or inhibiting the release of CGRP, a nociceptive neurotransmitter in DRG after nerve root injury.