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目的:研究骨巨细胞瘤细胞体外培养的生物学特性,并建立人骨巨细胞瘤细胞系.方法:采用原代组织块培养法培养骨巨细胞瘤手术标本,对存活细胞进行形态学观察、免疫组织化学染色、细胞周期检查、核型分析、裸鼠移植.结果:建立人骨巨细胞瘤细胞系GCT-0404,其形态学表现、免疫组织化学染色均符合骨巨细胞瘤纤维母细胞样基质细胞的特征.经过近1a的体外培养,现已传代100次,细胞倍增时间39.7h,细胞周期测定G1期为67.5%,G2期为8.9%,S期为23.6%.染色体具有三倍体核型.裸鼠移植成瘤率100%,无支原体污染.结论:人骨巨细胞瘤细胞系GCT-0404可以用于对骨巨细胞瘤的研究.
OBJECTIVE: To study the biological characteristics of human giant cell tumor cell in vitro and to establish a human giant cell tumor cell line.Methods: The primary cultured tissue samples were used to culture the giant cell tumor of the giant cell tumor, the morphological observation of the surviving cells, Histopathological examination and immunohistochemical staining of GCT-0404 cell line were all in line with those of giant cell tumor of fibroblastoid stromal cells After nearly 1a in vitro culture, it has been passaged 100 times, cell doubling time 39.7h, cell cycle determination of G1 was 67.5%, G2 period was 8.9%, S period was 23.6% .Thermal chromosome with triploid karyotype The rate of tumorigenesis in nude mice was 100%, with no mycoplasma contamination.Conclusion: Human giant cell tumor cell line GCT-0404 can be used in the study of giant cell tumor of bone.