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已知培养的丝虫能分泌乙酰胆碱酯酶(AchE),在感染过程中虫的AchE能使宿主产生抗AchE抗体。本试验首次证实感染旋盘尾丝虫的病人血清及感染彭亨丝虫的纳塔尔多乳鼠血清的冷PEG沉淀物有活性AchE。由感染乳鼠的心脏和肺血管以及血分别采集彭亨丝虫成虫及微丝蚴,培养24h后收集其排泄分泌物(ES)。旋盘尾丝虫结节取自利比亚病人,经0.5%胶原酶消化后得到成虫,在含0.1% Triton X-100的0.1mol/L磷酸钾缓冲液(pH7.4)中制成匀浆后100000g离心,上清作为虫体提取物。微丝蚴在同样缓冲液中冰浴超声后同上离心。鹿
It is known that cultured filarial silkworms can secrete acetylcholinesterase (AchE) which causes the host to produce anti-AchE antibodies during infection. This trial, for the first time, confirmed the presence of active AchE in the serum of patients infected with Onchocercus variabile and in the cold PEG precipitate from the serum of Natal poly-litter infected with Paenibacillus. Adult and microfilariae of Paenotherium penicilli were collected from the heart, pulmonary blood vessels and blood of the infected suckling mice, and secreted secretions (ES) were collected after culturing for 24 hours. The wormworm nodules were taken from Libyan patients and were digested with 0.5% collagenase to give adult worms and were homogenized in 0.1 mol / L potassium phosphate buffer (pH 7.4) containing 0.1% Triton X-100 100000g centrifugal, supernatant as parasites extract. Microfilaria was homogenized in the same buffer after ice bath sonication. deer