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In our previous work, naltrexone, a common opioid receptor antagonist was found to be able to inhibit transplantation rejection. The purpose of present study is to investigate the subtype of the opioid receptors involved in transplantation rejection.The in vivo experiment:Cardiac tissue of the newborn C57 mice was transplanted into the ear of Balb/c mice.The survival of the transplanted tissue was tested with the ECG of transplanted tissue. Naltrexone or naltrindole (a selective opioid receptor antagonist) 5 mg-kg~(-1) was injected ip twice per day for 10 d, respectively.The in vitro experiment: Lymphocytes from two different strain mice were mixed and cultured together. Naltrexone, naltrindole or enkephalin (a selective opioid receptor agonist) was added into the culture fluid, respectively.~3H-TdR uptake (cpm) was measured to show the mixed lymphocyte reaction.The mean
In our previous work, naltrexone, a common opioid receptor antagonist was found to be able to inhibit transplantation transplantation. The purpose of present study is to investigate the subtype of the opioid receptors involved in transplantation assay. Cardiac tissue of the newborn C57 mice was transplanted into the ear of Balb / c mice. survival of the transplanted tissue was tested with the ECG of transplanted tissue. Naltrexone or naltrindole (a selective opioid receptor antagonist) 5 mg-kg ~ (-1) was injected ip twice per day for 10 d, respectively. in vitro experiment: Lymphocytes from two different strains of mice were mixed and cultured together. Naltrexone, naltrindole or enkephalin (a selective opioid receptor agonist) was added into the culture fluid, respectively. -TdR uptake (cpm) was measured to show the mixed lymphocyte reaction. The mean