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本文报告一种检测天然矿泉水和瓶装水铜绿色假单胞菌的方法。方法中，直接在样品瓶中添加青霉素营养肉汤增菌，改良十六烷三甲基溴化铵平板划线分离，以菌落形态为主要判断指标，牛奶琼脂划线确证。全过程４２℃７２ｈ．与常用方法相比，本方法灵敏，简便，廉价，对干扰杂菌抑制效果明显，尤其适于铜绿色假单胞菌菌数较少并受到加工损伤的水样。应用此方法，８１个批号瓶装矿泉水样品检出１０份阳性。
This article reports a method for the detection of natural mineral water and bottled water Pseudomonas aeruginosa. In the method, the penicillin nutrient broth was directly added into the sample bottle to improve the stencil separation of cetyltrimethylammonium bromide, the colony morphology was the main judgment index, and the milk agar was confirmed by scribing. The whole process 42 ℃ 72h. Compared with the common methods, the method is sensitive, simple and cheap, and has obvious inhibitory effect on interfering bacteria, and is particularly suitable for water samples with less Pseudomonas aeruginosa bacteria bacteria and being damaged by processing. Application of this method, 81 batches of bottled mineral water samples detected 10 positive.