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以X-XOD体系产生O_2、Luminol作为发光增强剂,用化学发光法测定了12种黄酮单体清除O_2反应的速率常数(k_3),其k_3值在10~(-5)～10~(-6)(mol/L)~(-1)sec~(-1)之间。以k_3值的大小作为比较指标,对黄酮体的结构与清O_2活力之问的关系进行了探讨。同时又用化学发光法测定了这些黄酮试样在1mol/L浓度时对V_C-H_2O_2-Cu~(2+)-酵母多糖体系产生的·OH的抑制,显示出黄酮类化合物在清除O_2和·OH时的活性部位和作用机制并不完全相同,认为4’-OH和△~(2(3))双键是清O_2的主要活性部位,而B环邻二羟基(3’,4’-di-OH)则是清除·OH的关键功能团。
The rate constants (k_3) of 12 flavonoid monomers were determined by chemiluminescence method with O_2 and Luminol as luminescence enhancers in X-XOD system. The k_3 value of 10 was between 10 ~ (-5) and 10 ~ (- 6) (mol / L) ~ (-1) sec ~ (-1). Taking the value of k_3 as a comparative index, the relationship between the structure of flavonoids and the activity of O_2 was discussed. At the same time, the chemiluminescence method was used to determine the inhibitory effect of these flavonoids on · OH induced by V_C-H_2O_2-Cu ~ (2 +) - zymosan system at the concentration of 1mol / L. OH and the mechanism of action is not the same. It is considered that the 4’-OH and △ ~ (2 (3)) double bonds are the main active sites of O_2 and the ortho-dihydroxy (B, di-OH) is the key functional group to clear · OH.