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本文介绍采用体外培养技术,根据~3H标记的次黄嘌呤摄入疟原虫核酸的情况,测定药物抗疟作用的方法。体外培养采用改良的Nguyen-Dinh和Trager蜡烛缸法。恶性疟原虫是对氯喹有一级抗性的FCR_(8TC)株和敏感的洪都拉斯株。塑料微量板分24井和96井两种。在24井板中,先加入20μl含50%红细胞而原虫密度为0.2～0.3%的混悬液,然后用含血清和不同浓度药物的RPMI1640加至500μl;在96井板中,先加入20μl含10%红细胞而原虫密度为1%的混悬液,再用上述含药及血清的培养液加至200μl,分别置于37℃的蜡烛缸中连续培养48小时,并计数此时的原
This article describes the use of in vitro culture techniques, according to ~ 3H labeled hypoxanthine intake of malaria parasite nucleic acid situation, the determination of anti-malarial drugs approach. In vitro culture improved Nguyen-Dinh and Trager candle cylinder method. Plasmodium falciparum is a FCR_ (8TC) strain sensitive to chloroquine and a sensitive Honduran strain. Plastic microplate 24 well and 96 well two. In 24 well plates, 20 μl of suspension containing 50% erythrocytes and protozoal densities of 0.2-0.3% are added first and then to 500 μl with RPMI 1640 containing serum and different concentrations of drug; in a 96 well plate, 20 μl of 10% erythrocytes protozoa density of 1% of the suspension, and then the drug-containing serum and serum culture to 200μl, were placed in a candle jar at 37 ℃ for 48 hours of continuous culture, and counting the original